Search results for " centri"

Cercospora beticola Toxin Inhibits Vanadate-Sensitive H+ Transport in Corn Root Membrane Vesicles

1988

The effect of Cercospora beticola toxin on the transport of protons by vanadate-sensitive ATPase was studied with corn (Zea mays) root microsomal vesicles prepared by differential centrifugation, sedimentation through a sucrose cushion, and washing with Triton X-100 plus KBr. In these preparations, addition of ATP induced intravesicular H(+)-accumulation as evidenced by a rapid quenching of the fluorescence of 9-amino-6-chloro-2-methoxy acridine. This quenching was relatively unaffected by inhibitors of mitochondrial and tonoplast-type ATPases, but was strongly reduced by inhibitors of plasma membrane H(+)-ATPase. C. beticola toxin markedly inhibited ATP dependent H(+)-transport, and this e…

A Humanoid Social Robot Based Approach for Indoor Environment Quality Monitoring and Well-Being Improvement

2020

The indoor environmental quality (IEQ) monitoring inside buildings where people spend most of their time is essential for ensuring their well-being. Traditional approaches based on Building Automation and Control Systems consider buildings equipped with many different sensors. Unfortunately, the sensors are not always placed for taking the measurements at the right positions. Besides, users could feel a negative perception due to continuous supervision. The present work proposes an approach based on a social humanoid robot that monitors indoor environmental quality. It friendly interacts with occupants providing appropriate suggestions. Particularly, the social robot has been endowed with c…

2019

Recent studies on extracellular RNA raised awareness that extracellular vesicles (EVs) isolated from cultured cells may co-purify RNAs derived from media supplements such as fetal bovine serum (FBS) confounding EV-associated RNA. Defined culture media supplemented with a range of nutrient components provide an alternative to FBS addition and allow EV-collection under full medium conditions avoiding starvation and cell stress during the collection period. However, the potential contribution of serum-free media supplements to EV-RNA contamination has remained elusive and has never been assessed. Here, we report that RNA isolated from EVs harvested from cells under serum-replacement conditions…

Multi-virion infectious units arise from free viral particles in an enveloped virus

2017

Many animal viruses are enveloped in a lipid bilayer uptaken from cellular membranes. Since viral surface proteins bind to these membranes to initiate infection, we hypothesized that free virions may also be capable of interacting with the envelopes of other virions extracellularly. Here, we demonstrate this hypothesis in the vesicular stomatitis virus (VSV), a prototypic negative-strand RNA virus composed by an internal ribonucleocapsid, a matrix protein, and an external envelope1. Using microscopy, dynamic light scattering, differential centrifugation, and flow cytometry, we show that free viral particles can spontaneously aggregate into multi-virion infectious units. We also show that, f…

Improving the Translational Medicine Process: Moving Patients From "End-Users" to "Engaged Collaborators".

2019

Translational medicine works through the definition of unmet medical needs, their understanding and final resolution. In this complex and multi-disciplinary process patients have always been regarded as "end-users" or no more than "data provider." Considering that the translational practice is nowadays highly inefficient (i.e., large intellectual and economical resources are wasted with limited impact on people health) here we propose to reverse the process: start from patients, engage them, and keep them at the center. A new partnership needs to be formed between the patients and the health care professionals, as well as the treating physicians, to make the most out of the current "health …

Genesi geometrica delle volte centriche

1996

Un'analisi completa degli organismi voltati comporta lo studio delle molteplici componenti necessarie alla loro definizione: configurazione geometrica, sistema costruttivo, area storico-geografica di appartenenza, interpretazioni Stilistiche. L’obiettivo di questo studio è indirizzato all' analisi delle volte continue su pianta centrica definibili attraverso le leggi della geometria euclidea. L’indagine si pone all'interno dei confini relativi alla loro configurazione geometrica e il criterio adottato, svincolato da classificazioni di carattere tipologico o stilistico, propone l'identificazione di famiglie geometriche in base alla figura del poligono di base dell'impianto. Si può identifica…

Le politiche del lavoro e i servizi per l'impiego: eredità passate e opportunità di innovazione

2017

Il programma europeo della Garanzia Giovani rivolto ai giovani Neet rappresenta una potenziale opportunità di innovazione delle politiche attive del lavoro. Tuttavia, la sua attuazione in Italia è stata accolta con molta preoccupazione in ragione dello stato dei servizi pubblici per l’impiego, che nelle regioni del sud non sono mai riusciti ad abbandonare la logica burocratico-certificatoria del vecchio collocamento. Questo lavoro presenta i dati di una ricerca svolta in Sicilia, una fra le regioni italiane più problematiche, con riferimento sia alle condizioni del mercato del lavoro, sia ai rendimenti istituzionali in materia di politiche per il lavoro. Nonostante le premesse tutt’altro ch…

A new method to isolate lichen algae by using percoll® gradient centrifugation

2001

AbstractA rapid method to isolate intact functional algae from the lichens Evemia prunastri and Ramalina farinacea has been developed. This method is based on the use of Percoll® gradients after mechanical disruption of lichen thalli. Results obtained show that the algal preparations were virtually free of contamination by fungal hyphae. The purified algal cells were photosynthetically active and without symptoms of photoinhibition, which indicates their functional integrity. This method may be used for the isolation of intact algae from a broad range of lichen species.

Nuove relazioni tra tessuto urbano e agricolo nel Parco del Gugliotta a Piano Tavola a Carini

2014

A sud-ovest della città di Palermo, lungo la fascia a nord-est dellʼabitato di Carini, segnata da monte Colombrina e attraversata dal torrente Gugliotta, si estende tra città e campagna unʼampia zona, denominata Piano Tavola. Elementi differenti ne costituiscono i margini: la frangia urbana dei volumi che si attestano sullʼasse di corso Italia (a ovest), lʼuliveto sotto Pizzo Castellaccio (a est), la villa Dominici (a nord-est), lʼarea industriale e il tracciato ferroviario (a nord) e la cava in località Manostalla (a sud-est). La lettura del paesaggio evidenzia la pressione esercitata dal costruito verso lʼuliveto e la campagna, la presenza di strade che cingono il quartiere isolandolo e l…

Sedimentation properties of chitosomal chitin synthetase from the wild-type strain and the 'slime' variant of Neurospora crassa.

1989

Marked differences in the pattern of sedimentation of cellular structures were observed after isopycnic centrifugation of crude cell-free preparations from the Neurospora crassa wall-less 'slime' variant and mycelial wild-type strain. Kinetic studies of particle sedimentation showed that the various types of subcellular components, as revealed by turbidity, UV absorption, polypeptide patterns, and chitin synthetase activity determinations, sediment independently of one another. An important feature was the finding that chitin synthetase from 'slime' peaked at a median specific gravity of 1.1201 +/- 0.0036, whereas that from wild-type strain sedimented at a higher buoyant density (specific g…